NGS Core Center

What is NGS?

MOMA NGS Core Center

About


MOMA NGS Core Center provides its users with next generation sequencing data using instruments from Illumina Inc. with market leader technologies.

All our services are offered as non-profit for research groups and clinical departments affiliated with Aarhus University or Aarhus University Hospital.

The Core Center was established in January 2012 by Aarhus University Hospital, The Faculty of Health at Aarhus University, and scientists at MOMA.

Our team of highly trained personnel provides guidance and advice for sequencing projects.

Equipment

Our fully equipped lab, including automation using liquid handling robots, support all common sequencing applications as well as the development of novel methodologies and protocols.

Data analysis

As per request, MOMA will perform the first level of bioinformatics data analysis (e.g data cleanup, mapping to reference genome, generation of gene count tables) by highly dedicated staff.
If support for subsequent downstream analysis is needed, we refer to our collaborators at CONNECT -  Center for Clinical and Genomic Data.

Publication Policy

The use of the services provided by the NGS Core Center in publications requires acknowledgement of the center.
A suggested acknowledgement is:
Library preparation / Next generation sequencing was performed by the NGS Core Center, Department of Molecular Medicine, Aarhus University Hospital, Denmark.

Services

In production

  • Whole-Genome Sequencing (WGS)
  • Exome Sequencing (human)
  • Targeted small panel sequencing (see gene lists of available panels)
  • Custom prepared ready-to-sequence pools/libraries
  • Amplicon/16S sequencing
  • RNA sequencing
  • Sanger sequencing / Fragment analysis
  • Cell free DNA Analyses (cf/ctDNA)
Under development
  • Whole-Genome Bisulfite Sequencing (WGBS) 
  • Genome-wide CRISPR screen sequencing 
  • Single-cell sequencing (10x genomics) 

Instrumentation nextseq


Illumina Sequencing 
NovaSeq 6000
NextSeq 500
MiSeq

Please see www.illumina.com for additional information on performance and specifications.

Quality Control
Agilent Tapestation 4200
Qubit 3.0
BioTek Synergy LX Platereaders
Lunatic Unchained Labs Dropsense

Library preparation
PerkinElmer Sciclone G3 NGS – automated liquid handling system
Covaris E220 Evolution
10x Genetics Chromium Controller

Other
Applied Biosystems 3500 XL Series Genetic Analyzers


Typical Workflow

Please, see the document Sample Requirements and Data Analysis for further details.

Procedure

Please contact us for more information about our services and prices.

We will arrange a short meeting to discuss your project in detail. This will cover the approach, targeting, library preparation and data handling. Our scientists will assist you in identifying the design that fits your project best.
A customer contract including a time schedule is required before your samples can be processed.
After completion of the deep sequencing analyses, we will perform the initial data processing. The data will be handed over to you personally as agreed upon.

 

References

MOMA NGS Core Center has provided services to a number of different  institutions/departments.