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cosmx smi
Spatial Molecular Imager
CosMx® Spatial Molecular Imager
The CosMx SMI is a platform for profiling RNA and protein expression at single-cell and sub-cellular resolution, while maintaining spatial context. The CosMx SMI is a spatial multiomics platform for profiling FFPE and fresh frozen tissues, without destroying the tissue sample.
Some of the available assays include the CosMx WTX Human Whole Transcriptome Panel Containing >18,000 (human), the CosMx 6K Discovery Panel containing 6,000 genes (human), the CosMx Universal Cell Characterization Panel containing 1,000 genes (human or mouse), and the CosMx Immuno-Oncology Protein Panel containing over 64 protein targets (human). Furthermore, there is the possibility to add customized targets.
For a full overview of available assays see:
The CosMx SMI at MOMA Single-Cell and Spatial Core Facility, and tissue microarray cores marked with fields of view (FOV) for target quantification and visualization.
Profiling by Combining IHC and ISH
The CosMx SMI platform uses cyclic in situ hybridization chemistry for quantifying and visualizing RNA and protein in tissue samples. Using in situ hybridization techniques, detection probes or antibodies labeled with DNA readout domains are hybridized to RNA or protein targets in the tissue sample.
Using immunohistochemical techniques, fluorescently labeled antibodies are used to stain FFPE or fresh frozen tissues. Once tissue structures are visualized by the fluorescently labeled antibodies, groups of cells can be selected by the user as fields of view (FOV) measuring 0.5 x 0.5 mm.
RNA or protein targets are identified through multiple rounds of reporter probe binding and fluorescent imaging on the CosMx SMI instrument.
Sample requirements
- Compatible samples: FFPE or Fresh Frozen tissue.
- Tissue should be sectioned within 2 weeks of planned CosMx experiment.
- We recommend mounting tissue sections on TOMO slides.
- Recommended section thickness:
- FFPE: 5 µm
- FF: 5-10 µm (but instrument will only image the 5 µm closest to slide).
- Tissue should be mounted within the CosMx scannable area of 15 x20 mm, shown
with green in the figure below. - After sectioning, the slide should be baked at 37C for 2 hours, followed by
drying overnight at room temperature. - Once dried, the slide should be stored at 4C with desiccant.
Image provided by Bruker Spatial Biology.
contact
Please contact us for more information about our services and prices.
Contact
Kasper Thorsen
Manager Single-Cell & Spatial Core Facility MSc, PhD
Sara R. N. Jensen
Clinical Specialist Single-Cell & Spatial Core MLT, MSc(Eng)
